Plasmid

Part:BBa_K4810013:Design

Designed by: Theodoros Desiniotis   Group: iGEM23_Athens   (2023-10-11)


M13KE vector modified for production of M13 Phages detecting “Eicosane”


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5293
    Illegal BamHI site found at 6670
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3998
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence of pVIII protein consists of 222bp coding for 73 amino acids. First 23 amino acids are cleaved by a signal-peptidase inside E.coli cells. Thus in order to introduce the modified hexapeptide (or any peptide) in the N-terminal of the functional protein, the appropriate codons should be introduced from the codon #24 and onwards in the sequence of the pVIII protein.



Source

M13KE DNA (unmodified plasmid)


References